叶绿体基因组测序分析

叶绿体基因组测序分析

介绍：叶绿体中包含的所有遗传物质称为叶绿体基因组。叶绿体基因组包含大量的遗传信息，同时叶绿体基因组结构保守，除了反向重复区外所有基因均为单拷贝，几乎不存在（或很少受）旁系同源基因的干扰，同时几乎没有来自线粒体和核的外源DNA。此外，叶绿体DNA的核苷酸替代率适中，在应用上有很大的价值，而且叶绿体基因组编码区和非编码区分子进化速率差异较大（前者慢后者快），因此可分别为不同层级的系统学研究提供依据。

随着测序技术的发展，下一代测序技术（NGS）使基因组的测序不入了一个低成本及高通量测序的时代。基于叶绿体基因组的高度保守性及二代测序的高通量性，研究者可以不通过分离纯化叶绿体DNA，而直接从全基因组高通量测序数据中获取叶绿体基因组。根据我司数百例叶绿体基因组组装经验，只有极少数类群需要结合三代测序或一代测序补gap。

叶绿体基因组测序分析流程图

叶绿体分析主要内容

代表文章（#为我司技术人员为共同作者）：

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ependent species from Peucedanum praeruptorum[J]. Physiology and Molecular Biology of Plants, 2021: 1-13.Complete chloroplast genome sequencing support Angelica decursiva is an independent species from Peucedanum praeruptorum | SpringerLink