基因家族鉴定分析
研究人员主要关注一些重要的转录因子和酶家族,这些家族成员和生物的生长发育、逆境胁迫、初生代谢和此生代谢的合成、积累、化学修饰以及转运等生物过程息息相关。因此,对基因家族的系统研究,筛选出一个家族中的其关键功能的基因,并预测与之互作或具有调控关系的基因,可以为后期的深入实验验证提供理论指导。也为提高产量、抗性、品质或害虫防控等提供理论基础。
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基因家族鉴定分析
基因家族(Gene family),是来源于同一个祖先,由一个基因通过基因重复而产生两个或更多的拷贝而构成的一组基因,它们在结构和功能上具有明显的相似性,编码相似的蛋白质产物。同一家族基因可以紧密排列在一起,形成一个基因簇,但多数时候,它们是分散在同一染色体的不同位置,或者存在于不同的染色体上的,各自具有不同的表达调控模式。
研究人员主要关注一些重要的转录因子和酶家族,这些家族成员和生物的生长发育、逆境胁迫、初生代谢和此生代谢的合成、积累、化学修饰以及转运等生物过程息息相关。因此,对基因家族的系统研究,筛选出一个家族中的其关键功能的基因,并预测与之互作或具有调控关系的基因,可以为后期的深入实验验证提供理论指导。也为提高产量、抗性、品质或害虫防控等提供理论基础。
基因家族鉴定分析流程图
代表文章(#为我司技术人员为共同作者,@为致谢我司技术人员):
1. Du Z, You S, Zhao X, Xiong L and Li J (2022) Genome-Wide Identification of WRKY Genes and Their Responses to Chilling Stress in Kandelia obovata. Front. Genet. 13:875316. doi: 10.3389/fgene.2022.875316
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3. # Yan H, Hua M G, da Silva J A T, et al. Genome-wide identification and analysis of NAC transcription factor family in two diploid wild relatives of cultivated sweet potato uncovers potential NAC genes related to drought tolerance[J]. Frontiers in Genetics, 2378.
4. # Yu Z, Zhang G, da Silva J A T, et al. Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation[J]. BMC Plant Biology, 2021, 21(1): 1-17.
5. Gao C, Gao K, Yang H, et al. Genome-wide analysis of metallothionein gene family in maize to reveal its role in development and stress resistance to heavy metal[J]. Biological Research, 2022, 55(1): 1-13.
6. Li H, Guan H, Zhuo Q, et al. Genome-wide characterization of the abscisic acid-, stress-and ripening-induced (ASR) gene family in wheat (Triticum aestivum L.)[J]. Biological Research, 2020, 53(1): 1-16.
7. @ Si, C., He, C., Teixeira da Silva, J.A. et al. Metabolic accumulation and related synthetic genes of O-acetyl groups in mannan polysaccharides of Dendrobium officinale. Protoplasma (2021). https://doi.org/10.1007/s00709-021-01672-8
8. Wang T, Jia Z H, Zhang J Y, et al. Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome[J]. Plants, 2020, 9(10): 1350.
9. Wang G, Wang T, Jia Z H, et al. Genome-wide bioinformatics analysis of MAPK gene family in kiwifruit (actinidia chinensis)[J]. International journal of molecular sciences, 2018, 19(9): 2510.
10. # He C, Si C, da Silva J A T, et al. Genome-wide identification and classification of MIKC-type MADS-box genes in Streptophyte lineages and expression analyses to reveal their role in seed germination of orchid[J]. BMC plant biology, 2019, 19(1): 223.
11. # He C, da Silva J A T, Wang H, et al. Mining MYB transcription factors from the genomes of orchids (Phalaenopsis and Dendrobium) and characterization of an orchid R2R3-MYB gene involved in water-soluble polysaccharide biosynthesis[J]. Scientific reports, 2019, 9(1): 1-19.
12. # Guo J, Duan H, Xuan L, et al. Identification and functional analysis of LecRLK genes in Taxodium ‘Zhongshanshan’[J]. PeerJ, 2019, 7: e7498.
13. # Su T, Han M, Min J, et al. Genome-Wide Characterization of AspATs in Populus: Gene Expression Variation and Enzyme Activities in Response to Nitrogen Perturbations[J]. Forests. 2019, 10(5): 449.
14. @ Jin X, Cao D, Wang Z, et al. Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses[J]. Scientific reports, 2019, 9(1): 1-15.
15. # Yan H, Zhang Y, Xiong Y, et al. Selection and Validation of Novel RT-qPCR Reference Genes under Hormonal Stimuli and in Different Tissues of Santalum album[J]. Scientific Reports. 2018, 8(1): 17511. IF= 4.122
16. # He C, Teixeira Da Silva J A, Tan J, et al. A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale[J]. Scientific Reports. 2017, 7(1). IF= 4.122